Many studies have revealed that circRNAs have unique expression signatures and contribute to biological processes through a variety of functions, for example, acting as transcriptional regulators/microRNA(miRNA) sponges/protein templates, and serving as protein decoys, scaffolds and recruiters etc. The unique structure and broad expression of circRNAs makes them potential candidates for diagnostic biomarkers and therapeutic targets of a variety of diseases, such as cancers, cardiovascular diseases, neurological disorders and autoimmune diseases etc. More recently, an increasing number of investigations have been involved in the field of circRNA.

Our Capability

The gain-of-function and loss-of-function are approaches to investigate the roles and mechanisms of circRNAs. Cell lines stably overexpressing circRNAs are useful tools for the functional characterization of circRNAs of interest. Creative Biogene has excellent platforms for providing products and services in the field of stable cell lines and circRNAs. So far, we have developed a large number of cell lines stably overexpressing various types of genes, premade circRNA molecules and expression vectors, with more under development. Now Creative Biogene is providing a comprehensive portfolio of circRNA stable cell lines from circRNA design, vector construction, to stable cell line generation and validation. All our circRNA stable cell lines are fully validated by sequencing and real-time qPCR to make sure the sequence accuracy and high expression level.

Highlights

• Overexpression of circRNAs in a broad type of cancer and immortal cell lines
• Stable and long-term expression of circRNAs
• Cell line models for evaluating gain-of-functions of circRNAs
• High overexpression level of circRNAs
• Good stability of circRNA overexpression
• Fully verified by QPCR and sequencing
• Mycoplasma free circRNA cell line models