Creative Biogene is a leading biotechnology company offering the best Lactic acid bacteria genome editing services. With years of experience and expertise in microbial genome editing, our talented scientists will work closely with you to provide any help in Lactic acid bacteria genome editing services.

Lactic acid bacteria (LAB) is a functionally related group of bacteria comprising the families Aerococcaceae, Carnobacteriaceae, Enterococcaceae, Lactobacillaceae, Leuconostocaceae and Streptococcaceae, which are phylogenetically diverse. They have a highly fermentative lifestyle, converting a range of sugars into mainly lactic acid. LAB play an important role in different forms of food-related biotechnology. They are gaining attention towards novel uses due to their safety for human and animal consumption, metabolic versatility and wide ecological niche adaptation (including industrial-scale fermentations).

Our Lactic acid bacteria (LAB) genome editing services are based on CRISPR/Cas9 technology and homologous recombination technique. The state-of-art Lactic acid bacteria (LAB) editing system helps you successfully achieve gene knockout, gene insertion and point mutation for either research or industrial purposes.

Red/ET Recombination permits the engineering of DNA in LAB using homologous recombination mediated by phage protein pairs, either RecE/RecT or Reda/Redb. The central step in Red/ET recombination is the crossover step between a targeting construct containing homology arms and the target which can be a gene locus on the LAB chromosome by designing a homologous fusion fragment of the target gene, it is cloned into a suicide vector, and the suicide vector is transformed into the target LAB. An insertion mutant is selected by antibiotic screening. Under the second round of reverse selection pressure, only the mutation that contain second homologous recombination and the loss of the suicide plasmid can survive. By PCR screening and sequencing, we can obtain the mutant of the strain.