CRISPR as a highly efficient gene editing tool can manipulate the genome of a cell to achieve gene knockout, knock-in, targeted gene mutation in specific cells. Gene-edited cell lines generated by CRISPR/Cas9 technology can be widely used for gene function research, signaling, recombinant protein preparation, drug discovery, and disease mechanism of action research. Creative Biogene's scientists utilize advanced CRISPR technology to efficiently and precisely modify specific genes in a variety of cell types, including but not limited to mammalian cells, stem cells, and other cells, based on the research needs of our clients. We are committed to providing comprehensive and efficient gene editing cell line generation services to accelerate your research.

Our project experts will work with you to develop customized gene-edited cell line generation strategies and provide one-stop cell line generation services. First, our experts will evaluate the cell lines you are interested in. The aim of the target evaluation is to assess the feasibility of gene knockout or knock-in for the target gene, as well as to test the transfection efficiency and monoclonal growth ability of the target cells in preliminary experiments. The process involves designing multiple sgRNAs, constructing vectors, and selecting sgRNAs with high editing efficiency through experiments conducted on cells. The next step is to introduce CRISPR vectors or RNP into recipient cells through electro- or viral transfection. Monoclonal screening and validation are then carried out, which includes sequencing to confirm gene knockdown at the genetic level (the default method), as well as optional methods such as FACS and WB to validate knockdown at the protein level. Finally, stable cell lines that have undergone gene editing are provided, ensuring they are mycoplasma-tested and free from contamination.